SMARCA4 marks developmental enhancers in an active or repressed state. (A) Intersection of forebrain SMARCA4-enriched regions with 1747 published mouse sequences tested in the transgenic assay shows that SMARCA4 marks enhancers and the histone signature at SMARCA4-enriched loci predicts tissue-specific activity. (Positive) Reproducible enhancer activity in forebrain; (other) reproducible enhancer activity in a tissue other than forebrain; (negative) no detectable enhancer activity in vivo. (B) Representative examples of regions with characteristic forebrain and limb chromatin state showing predicted tissue specificity in transgenic assays. One representative embryo is displayed for each region and the reproducibility of the observed pattern is indicated (number of observed/total number of embryos) for each. The predicted activity in forebrain (fb) is shown both in the side views (red arrows) and top views (the angle from which the image was captured is indicated on the side views by a black arrow).
