Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

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Figure 5.
Figure 5.

Off-target mutations caused by RGEN RNPs vs. RGEN plasmids. RGEN RNPs or plasmids that encode Cas9 and sgRNA were electroporated into K562 cells. Mutations were detected using the T7E1 assay (left) and deep sequencing (right). The PAM sequence is shown in blue. Mismatched bases are shown in red.

This Article

  1. Published in Advance April 2, 2014, doi: 10.1101/gr.171322.113 Genome Res. 24: 1012-1019

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