Figure 4.
Targeted chromosomal deletions via RGEN RNPs. (A) RGEN target sites in the region of the CCR5 locus. The distances between the CCR5 site and each of the other sites are shown. Arrows indicate PCR primers. Red arrowheads indicate sgRNA target sites. (B) PCR products corresponding to deletions in K562 cells treated with RGEN RNPs; 15 μg of Cas9 protein premixed with 20 μg each of two sgRNAs was transfected into 2 × 105 K562 cells. (C) DNA sequences of deletion-specific PCR products. In cases in which a sequence was detected more than once, the number of occurrences is shown in parentheses.
