Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

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Figure 3.
Figure 3.

Homology-directed repair using ssODNs. The 86-mer ssODN includes an XbaI restriction enzyme site, which is absent at the target site, between two short homology arms. PCR amplicons were digested with XbaI in an RFLP assay to detect sequences that resulted from homology-directed repair.

This Article

  1. Published in Advance April 2, 2014, doi: 10.1101/gr.171322.113 Genome Res. 24: 1012-1019

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