Figure 2.
Genome editing in BJ fibroblasts and H9 hES cell lines via direct delivery of RGEN RNPs. (A) CCR5-specific RGEN-driven mutations detected by the T7E1 assay in H9 and BJ cells. (B) RGEN-driven mutations in H9 ES cells detected by the T7E1 assay. A mixture of Cas9 protein (75 μg) and sgRNA (100 μg) was transfected into 1 × 106 H9 cells. (C) Cytotoxicity of RGEN RNPs vs. RGEN plasmid in H9 ES cells. (**) P < 0.01, (*) P < 0.05. (D) No apparent changes in the physiology of ES cells after RGEN RNP treatment. Untransfected, RNP-, and plasmid-transfected ES cell colonies were subjected to AP staining.
