Figure 1.
Targeted mutagenesis in human K562 cells via direct delivery of RGEN RNPs. (A) CCR5-specific RGEN RNP-mediated mutations measured by the T7E1 assay. (B) Mutant DNA sequences at the CCR5 locus. The 20-bp target sequence is underlined and shown in bold. The PAM sequence is shown in red. (C) RGEN RNP-mediated mutagenesis at several endogenous loci. A mixture of Cas9 protein (15 μg) and sgRNA (20 μg) was transfected into 2 × 105 K562 cells. PCR amplicons around RGEN target sites were subjected to the T7E1 assay. Representative data from at least three independent experiments are shown.
