Exon skipping divergence is influenced more by differences in trans-acting factors than other classes of alternative splicing. (A) Percentage of alternative splicing events affected by cis- and trans-acting regulatory divergence for two F1 hybrid crosses. Skipped exons (SE) have many more events affected by trans-regulatory divergence. (B) Relative contribution of cis-regulatory divergence in divergent alternative splicing. The fraction of species-specific splicing differences attributable to cis-acting changes (% cis) is plotted for four classes of alternative splicing. Exon skipping has a much lower contribution from cis-acting changes, reflecting a higher contribution of trans-regulatory divergence. P-values indicate the results of Wilcoxon rank sum tests comparing the mean % cis of different alternative splicing types.
