TP73 gene body CGI hypomethylation associated with activation of the oncogenic DeltaN TP73 isoform. (A) Strong focal hypomethylation in GBM 3 detected by MeDIP-seq and MRE-seq. (B) Bisulfite sequencing for two normal brains (top) and GBMs 1 and 3 (bottom). Asterisks indicate CpGs with MRE sites. (C) Infinium HumanMethylation27 array data at the DeltaN TP73 promoter. Percent methylation for four CpGs, shown schematically at the top, is graphed below. (Gray points) 292 TCGA-assayed GBMs. (Black squares) GBM 3. (Black triangles) Normal brains. (D) Quantitative RT-PCR for the oncogenic DeltaN TP73 mRNA isoform. Fold expression ± SD relative to the average of two normal brains is graphed for a representative experiment. (NB) Normal brain. (FNSC) Cultured fetal neural stem cells. (U87) GBM cell line. (GBM5 cult.) Short-term serum-grown culture of GBM 5. (E) Western blotting with anti-deltaNp73 (top) and anti-GAPDH control (bottom).
