H3K4me1 is enriched at distal p300 peaks in Nematostella. (A,B) Gastrula (A) or planula (B) p300 peaks were split into peaks >300 bp distal to transcription start sites (TSSs) (distal, left) and peaks within 300 bp around TSSs (TSS, right). On the y-axis, the enrichment of H3K4me1 (blue boxes) or H3K4me3 (gray boxes) normalized to input is plotted. Distal p300 peaks have higher H3K4me1 than H3K4me3 levels, a characteristic of enhancers. As expected, p300 peaks around TSSs are more enriched in H3K4me3. (C) Peaks of three different transcription factors (Reb1, Gal4, Phd1) derived from ChIP-exo experiments (Rhee and Pugh 2011) in Saccharomyces cerevisiae were split into distal and TSS overlapping peaks as in A and B. A total of 70% of peaks overlapped TSSs, and the remaining peaks were still within 2 kb around the TSS. On the y-axis, the enrichment of H3K4me1 (blue boxes) or H3K4me3 (gray boxes) normalized to input (Kirmizis et al. 2007) is plotted. P-values were calculated using the Wilcoxon rank sum test.
