Restless Legs Syndrome-associated intronic common variant in Meis1 alters enhancer function in the developing telencephalon

Motor restlessness/hyperactivity in adult heterozygous Meis1^tm1Mtor mice. Heterozygous Meis1^tm1Mtor (het) traveled a higher total distance (P = 0.003, two-tailed t-tests, n = 29) (A) and moved with a higher speed on average (P = 0.004, two-tailed t-tests, n = 30) (B) in a 20-min open field test for spontaneous locomotor activity in a novel environment. (C) Oxygen consumption (mL/h) plotted versus body mass (g). Scatterplot with regression lines split by sex and genotype to adjust for body mass variation. The shift in regression lines indicates higher energy turnover in heterozygous Meis1^tm1Mtor mice (linear regression model, P = 0.0005). (D) Mean distance traveled (cm/20 min) monitored by infrared light beams during the indirect calorimetry trial. Heterozygous Meis1^tm1Mtor mice tended to show increased locomotor activity under home cage conditions (two-way ANOVA, P = 0.06).