Conserved and diverged sequence features of vertebrate H3K27me3 domains. (A) The top panel shows the predicted nucleosome occupancy of H3K27me3 domains (red line) and random genomic regions (gray line). The maximum nucleosome occupancy (van der Heijden et al. 2012) was calculated per 1 kb using a moving window (window size, 1 kb; step size, 100 bp). Shown is the mean of predicted maximum occupancy for all H27me3 domains or random genomic regions. The bottom panel shows the median ChIP-seq enrichment of H3K27me3 domains. (B) %GC of H3K27me3 domains and genomic background in human, Xenopus tropicalis, and zebrafish. (C) Overrepresentation of dinucleotides in human, X. tropicalis, and zebrafish H3K27me3 domains compared with the genomic background (log2 of the fold difference in dinucleotide frequency).
