Figure 8.
Mismatch distribution along the reads. (A) AG/TC sites are evenly distributed along the reads and are even slightly depleted toward the read ends, as the alignments are more sensitive to mismatches in this region. (B) Other types of mismatches (GA/CT) show a pronounced increase toward the read ends, suggesting many of these mismatches, albeit trimming, could be attributed to alignment artifacts. Reads are 75 bp long.
