Characterizing RUNX1–RUNX1T1 balanced translocation in Kasumi-1. Lanes 1, 2, 4, 6, and 8 contain 1 kb of Plus GeneRuler DNA ladder, PCR products from Kasumi-1 Der8 with all 28 primers (3.5 kb), 14 primer FE ∪ RO (3.5 kb), 14 primer FO ∪ RO (6.8 kb), and 14 primer FO ∪ RE (10.1 kb). Lanes 3, 5, 7, and 9 contain matching water controls, which show no contamination. Lanes 10, 12, 14, and 16 contain PCR products from Kasumi-1 Der21 with all 29 primers (2.7 kb), 15 primer FO ∪ RO (2.7 kb), 15 primer FE ∪ RO (6.1 kb), and 14 FE ∪ RE (8.1 kb). The gel was loaded with 2 μL for lanes 2–5 and 10–13, and 4 μL for remaining volumes. Reactions with shorter amplicons amplified extremely well, and lesser volumes were used for visualization on the gel. The expected amplicon lengths according to the Der8 and Der21 design are listed in parentheses.
