Amplification and thrifty single-molecule sequencing of recurrent somatic structural variations

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Figure 5.
Figure 5.

Subsampling of nine primers from the complete AMBRE-68 tiling design results in clean amplification of CDKN2A loss DNA fragments in six cell lines. (From left to right) Lanes contain 1 kb of Plus GeneRuler DNA ladder, PCR products from samples A549 (2.2 kb), CEM (5.8 kb), MCF7 (3.6 kb), MOLT4 (6.8 kb), T98G (7.5 kb), HEK, and water. The expected lengths of each amplicon according to AMBRE-68 design are listed in parentheses. HEK cells (no CDKN2A deletion) and H2O are negative controls.

This Article

  1. Published in Advance December 4, 2013, doi: 10.1101/gr.161497.113 Genome Res. 24: 318-328

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