Figure 3.
Cnot2 expression suppresses tumor progression. (A) Overexpression of Cnot2 in 6DT1 cells showed 22% reduction in primary tumor mass (P = 0.021) and 46% reduction in pulmonary metastases (P = 0.006, n = 20 per group). (B) Short hairpin-mediated knockdown Cnot2 by 51% (sh62) and 42% (sh64) in 6DT1 cells resulted in no significant change in primary tumor mass but enhanced metastatic potential respectively by 183% (P = 0.002) and 87% (P = 0.026, ncontrol = 18 mice, nsh62 = 10 mice, nsh64 = 9 mice). (C) Representative lungs from knockdown experiment sectioned and stained with hematoxylin and eosin (H&E). (*) P < 0.05, (**) P < 0.01.
