A transcriptional module centered on Cnot2 shows regulation by Cnot2 expression and predicts outcome in human breast cancer cohorts. (A) The Cnot2 module. Circles indicate individual genes. Connections between the genes were generated by minimizing the number of connections necessary to explain the gene expression correlations. (B) The effect of Cnot2 overexpression in 6DT1 cells on network hub transcripts. (*) P < 0.05, (**) P < 0.01. (C) A gene signature generated from homologous Cnot2 module hub transcripts predicts survival in human breast cancer cohorts. (D) Oncomine data set (Finak et al. 2008) shows CNOT2 is down-regulated in invasive breast carcinoma relative to normal breast tissue (normal breast tissue n = 6, invasive breast carcinoma n = 53; fold change = −16.3; P = −1.12 × 10−24).
