Figure 3.
Clones A11 and E9 are fully haploid human cell lines. (A) Deletion PCR (for experimental details, see Fig. 2B) for clones A11 and E9, as well as HAP1 wild-type cells. (B) To assess loss-of-heterozygosity in clones A11 and E9, we isolated genomic DNA and selected five genomic loci containing SNPs that were heterozygous in HAP1 cells. Each locus was amplified by suitable PCR primers and the PCR products were sent for Sanger sequencing. (C) Clones A11 and E9 were analyzed by spectral karyotyping to assess the global genomic landscape of these clones.
