Genomic makeup of HAP1 and strategy for deletion of the Chromosome 15 fragment. (A) HAP1 cells were subjected to spectral karyotyping to characterize their chromosomal landscape and identify disomic regions. (B) Single-nucleotide polymorphism data from KBM-7 cells were analyzed to identify chromosomal segments that are heterozygous and hence disomic. (Top) Heterozygous SNPs are depicted in red; homozygous SNPs, in turquoise. (Bottom) B-allele frequency (BAF) on Chromosome 15. A BAF of 0.5 is indicative of heterozygosity. (C) Schematic representative of the gRNA design. Two gRNAs (colored in pink) were placed at the boundaries of the disomic region from Chromosome 15.
