Figure 4.
Tagmentation reactions with molecular crowding agents. (A) Bioanalyzer electropherograms of tagmented DNA from reactions with different concentrations of PEG 8000 or no PEG or using Nextera XT reaction conditions. (B) Bioanalyzer electropherograms of tagmented DNA using PEG polymers of different MWs. (C) Representative Bioanalyzer electropherograms of tagmented DNA used to optimize the concentration of PEG 8000 in tagmentation reactions and to demonstrate how tagmented fragment size can be modulated by PEG amounts. (A–C) All reactions were performed on 100 pg HEK293T cell cDNA together with 12 (A) or 13 (B,C) cycles of enrichment PCR.
