Comparable single-cell gene expression profiles with in-house Tn5. (A) Percentage of genes reproducibly detected in replicate cells, binned according to expression level. We performed all pairwise comparisons within replicates for the libraries generated with Nextera protocol or five modified protocols exchanging different procedure components (Supplemental Table 1) and report the mean and 90% confidence interval. (B) Standard deviation in gene expression estimates within replicates for Nextera or five variant protocols (as in A) in bins of genes sorted according to expression levels. Error bars, SEM (n ≥ 4). (C,D) Comparison, as in A and B, for sequence libraries generated with identical reaction buffers (Supplemental Table 1) but with either produced or commercial Nextera Tn5 transposase.
