Figure 2.
Efficient and robust tagmentation reactions. (A) Bioanalyzer electropherograms of tagmented DNA libraries using Nextera (with column purification), in-house Tn5, and buffers with and without column purification. (B) Bioanalyzer electropherograms of tagmented DNA libraries generated with in-house Tn5 that were stripped with different amounts of SDS. (C) Fraction of sequenced reads mapping to DNA ladder sequences of varying lengths. The fraction of reads was adjusted for molarity of DNA ladder sequence, as determined with Bioanalyzer (Agilent).
