Figure 1.
The evolution experiment of the Saccharomyces cerevisiae haploid Δmsh2 strain. We started five independent evolution experiments from a single ancestral colony. Every 48 h, we randomly picked one colony and transferred it to another plate. The experiment involved 100 passages, corresponding to roughly 2200 generations (G) of S. cerevisiae. Single colonies were isolated for genome sequencing at passages 20, 30, 50, 70, 90, and 100, and growth was assayed at those time points of the experiment. Every 10 passages, a single colony was frozen at −80°C, obtaining a yeast fossil record.
