Stability of inheritance of CG-DMPs accumulated during multigenerational propagation on saline soil. Comparisons of methylation status at CG-DMPs in genomes of G10 and G11 plants. (A) IGV views of methylation level differentials. Column height indicates the relative extent of methylation at individual CG positions in G1 (black), control G10 (green), saline soil G10 (dark red), and saline soil G11 (red) genome samples in a specific region of chromosome 1. The blue arrows highlight example CG-DMPs where methylation has been lost in one of the saline soil G10 samples (G10-S2). Absence of methylation at these CG-DMPs is retained (stably inherited) in the subsequent generation, in the G11-S2 genome sample (see Supplemental Fig. S3). (B) Overall frequencies of retention of methylation status in G11 plants. CG-DMPs identified in G10 plants (G10-S2 or G10-C1) are shown as Retained (unchanged methylation status [stably inherited] in G11-S2 or G11-C2) or as Not Retained (where methylation status differs between G10 and G11 genomes).
