A subset of H3S28ph target genes is primed for later activation. (A) Western blot analysis of H3S28ph levels in serum deprived mouse Swiss 3T3 fibroblasts treated with anisomycin for 1, 3, and 6 h. H3 C-terminus antibody was used as a loading control. (B) Barplot showing the overlap between genes associated with H3S28ph after 1 h of anisomycin treatment and genes up-regulated after 1, 3, and 6 h after stress induction. P-values were calculated using a hypergeometric test. (C) ChIP-qPCR analysis of stress-induced H3S28ph at Optn and Ube2v2 genes in control (C) and anisomycin-treated cells (A) in the absence or presence of H89. Error bars represent SDs (n = 3). (*) P < 0.05. (D) RT-qPCR analysis of Optn and Ube2v2 gene expression after 1, 3, and 6 h of anisomycin treatment in the absence and presence of H89. Error bars represent SDs (n = 3). (*) P < 0.05.
