
siteRNA clusters are enriched for repressive H3K9me3 and H4K20me3 epigenetic marks. ChIP-qPCR profiles for tri-methylated H3K4 (H3K4me3), H3K9me3, and H4K20me3 histone modifications at the TSS and siteRNA cluster region. Chromatin was prepared from whole X. tropicalis embryos at stages 10 and 18. The quantity relative to the input after qPCR was calculated for each IP. The bars marked “beads” correspond to a control IP without antibody. Means and standard deviations of duplicates from a representative experiment are shown.











