Figure 7.
KDM4C overexpression accelerates cell growth. (A) Overexpression of KDM4C in primary fibroblasts was confirmed by qRT-PCR and Western blot relative to control cells. (B) Target gene expression changes following KDM4C overexpression in primary fibroblasts (RPS6 is used as a control). (C) Cell growth following KDM4C overexpression was measured by growth curve analysis (measured in quadruplicate; P = 0.003, ANOVA). (D) BrdU incorporation was measured in triplicate relative to control cells ([*] P < 0.05, t-test). (E) Changes in the proportion of cells at different stages of the cell cycle following KDM4C overexpression. Ctr indicates control cells. Error bars, SEM.
