Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

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Figure 7.
Figure 7.

Paired Cas9 nickases do not induce translocations. (A) Schematic overview of chromosomal translocations between the on-target and off-target sites. (B) PCR amplification to detect chromosomal translocations. (C) Translocations induced by Cas9 nucleases but not by the nickase pair.

This Article

  1. Published in Advance November 19, 2013, doi: 10.1101/gr.162339.113 Genome Res. 24: 132-141

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