Figure 6.
DNA splicing induced by paired Cas9 nickases. (A) The target sites of paired nickases in the human AAVS1 locus. The distances between the AS2 site and each of the other sites are shown. Arrows indicate PCR primers. (B) Genomic deletions detected using PCR. Asterisks indicate deletion-specific PCR products. (C) DNA sequences of deletion-specific PCR products obtained using AS2 and L1 sgRNAs. Target site PAM sequences are shown in red, and sgRNA-matching sequences are shown in capital letters. Intact sgRNA-matching sequences are underlined. (D) A schematic model of paired Cas9 nickase-mediated chromosomal deletions. Newly synthesized DNA strands are shown in red.
