Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

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Figure 5.
Figure 5.

Comparison of Cas9 nuclease and nickase behavior. (A) On-target mutation frequencies associated with Cas9 nucleases (WT), nickases (D10A), and paired nickases. Paired nickases that would produce 5′ overhangs or 3′ overhangs are indicated. (B) Analysis of off-target effects of Cas9 nucleases and paired nickases. A total of seven potential off-target sites for three sgRNAs were analyzed.

This Article

  1. Published in Advance November 19, 2013, doi: 10.1101/gr.162339.113 Genome Res. 24: 132-141

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