Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

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Figure 2.
Figure 2.

Mutation frequencies at CCR5 on-target sites and CCR2 off-target sites. K562 cells were transfected with RGENs that target nine different sites. PCR amplicons that span the on-target and off-target sites were subjected to deep sequencing.

This Article

  1. Published in Advance November 19, 2013, doi: 10.1101/gr.162339.113 Genome Res. 24: 132-141

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