Figure 5.
Germline transmission and mutant phenotypes of the Prkdc mutant mouse. (A) Detailed genotypes of F0 mutants (♂ 25 and ♀ 33, upper panel) and determination of genotypes of their F1 progenies (#1 and #7) by fPCR (lower panel). Note that a mutant allele (+2) of female founder #33 was only detected by fPCR (Supplemental Table 2). Using lymphocytes isolated from peripheral blood (PBMC), lymph node (LN), spleen, and thymus, frequencies of CD4+ and CD8+ T cells (B) and those of CD3+ T cells and CD19+ B cells (C) were determined. The abnormal frequencies of immune cells are denoted in red. BALB/c and scid mice, positive and negative controls for B and T lymphocytes, respectively.
