Figure 4.
Analysis of off-target activity of RGENs in Prkdc-mutant founders. T7E1 assays were conducted using genomic DNA samples from the indicated Prkdc mutant founders. The Prkdc gene and putative off-target sites bearing 1 to 3-bp mismatches that were selected with Bowtie 0.12.9 (Langmead et al. 2009) were examined. Bi-allelic mutants containing large insertions are shown in blue (Supplemental Table 2), mismatched bases in red, and PAM in green; black lines indicate putative 11-bp seed regions.
