Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 3.
Figure 3.

RNA-guided mutagenesis in zebrafish embryos. (A,B) Target sequences (upper) and RGEN-induced indels detected by T7E1 assays (lower). The Cas9 protein–sgRNA complex was microinjected into one-cell-stage embryos with concentrations of 1 ng, 4 ng, or 8 ng per embryo. (C) Time courses of Cas9 protein:sgRNA and Cas9 mRNA:sgRNA injection into embryos during early development. Arrows indicate bands cleaved by T7E1. (hpi) Hours post-injection.

This Article

  1. Published in Advance November 19, 2013, doi: 10.1101/gr.163394.113 Genome Res. 24: 125-131

Preprint Server



Current Issue

  1. January 2026, 36 (1)
  1. Current Issue
  1. Alert me to new issues of Genome Research