Figure 2.
RGEN-induced Prkdc gene disruption in mice. (A) Representative T7E1 assays demonstrating mutagenesis efficiencies of Cas9 mRNA plus Prkdc-specific sgRNA that were delivered via intracytoplasmic injection into one-cell-stage mouse embryos. Numbers indicate independent founder mice generated from the highest dose. Arrows indicate bands cleaved by T7E1. fPCR results (B) and DNA sequences of mutant alleles (C) observed in three Prkdc mutant founders denoted in red in A. The red arrowhead in C indicates the cleavage site by the RGEN.
