Experimental design facilitates high-dimensional phenotyping. A schematic of the experimental design used to obtain phenotypic data for the 22 strains. Icons next to strains show examples of sources from which strains were isolated (Supplemental Table 1). The schematic outlines the process of obtaining phenotype data for a single strain. In total (aggregated across all strains), we obtained 16 Gb of DNA sequence; 820 million RNA-seq reads; 912,000 mass spectra that we used to infer peptide levels; metabolic measurements of molecules in 40 different biochemical pathways; and 2000 images that together captured 21,000 cells, whose morphological characteristics we tabulated.
