Analysis of t(11;22)(q24;q12) breakpoint junctions. (A) Deletion lengths for der(11) and der(22) junctions from ZFN-induced translocations from hES-MP cells. Each value represents the combined deletion from both ends of an individual junction. The median deletion length is indicated by a bar on the graph, and the value is given below the graph. (B) Microhomology length distributions for junctions from ZFN-induced and tumor translocation (Zucman-Rossi et al. 1998). [Der(11) and der(22) are grouped.] Only junctions with simple deletions (i.e., without an insertion) are included. The probability that a junction will have X nucleotides of microhomology by chance assumes an unbiased base composition and is calculated as previously described (Roth et al. 1985). (C) Examples of complex breakpoint junctions with insertions derived from sequences near the DSB sites. Schematics of the derivative and parental chromosomes are shown. The DSB sites on the unrearranged chromosomes are represented by scissors. Segments that are duplicated, deleted, or added are represented as independent blocks and in the orientation relative to that found on the parental chromosomes (see Supplemental Fig. S4 for the sequences).
