mRNA expression is dysregulated by the deletion of MSs in UTRs. (A) The number of mononucleotide repeat tracts in genes that correlate with down-regulated mRNA expression were identified by comparing the results of whole-genome sequencing with those of RNA sequencing in three MSI-H cell lines. (B) mRNA transcript levels of MGLL with MSIs within their 3′ UTR in MSI-H cell lines were measured by Q-PCR. The levels of mRNA expression quantified by RNA sequencing are shown. (C) A schematic of the luciferase gene construct is displayed above. Significant deletion of polyA in the 3′ UTR causing decreased mRNA stability was confirmed via a reporter assay in MKN-1 cell line.
