Figure 1.
Examples of proximal enrichments resulting from ChIP-seq. (A) ChIP-seq of a transcription factor (green) results in immunoprecipitation of bound DNA sequence (blue) as well as addition of DNA sequence (orange) in close proximity. Only bound sequence shows evidence of DNase I footprint and binding motif. (B) Immunoprecipitation of DNA sequence associated with large multiprotein complex results in artifactual indirect enrichments for a wide range of transcription factors. (C) Active enhancers exhibit a range of ChIP-seq enrichments as a result of a close spatial proximity to histone modification and transcription factors at promoters.
