(A) Motif identified by MEME motif-finding algorithm from CEBPB ChIP-seq peaks before ATF4 induction. (B) Percent of methylated 8-mers with one CG dinucleotide in CEBPB ChIP-seq peaks in dermal fibroblasts plotted against Z-scores obtained from protein binding microarrays of CEBPB-GST binding to methylated 8-mers. The 8-mers are color coded: CG (gray dots), TGAC|G (square), TTGC|G (black dots). (C) Enriched GO terms for genes bound by CEBPB in dermal fibroblasts with methylated and unmethylated CEBP canonical motif (TTGC|GCAA) within −10 kbp to 1 kbp of the TSS. (D) CEBPB binding to unmethylated and methylated CEBP 10-mer palindromes in primary female mouse dermal fibroblasts. (E) Western blot showing induction of ATF4 in mouse dermal fibroblasts after 3 h of treatment with 2 μM thapsigargin (Tg). (F) UCSC Browser shot of CEBPB and ATF4 ChIP-seq read coverage before and after ATF4 induction along with the percent methylation at each CG dinucleotide in primary mouse dermal fibroblasts. CEBPB and ATF4 are preferentially localized in the methylated regions only after ATF4 induction. (G) Motif identified by MEME from new CEBPB ChIP-seq peaks after treatment with Tg.
