CG methylated microarrays identify a novel methylated sequence bound by the CEBPB|ATF4 heterodimer that is active in vivo

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Figure 4.
Figure 4.

(A) EMSA showing CEBPB|ATF4 heterodimer preferentially binds to methylated CGAT|GCAA. Purified CEBPB, ATF4, and CEBPB|ATF4 B-ZIP domain dimers were mixed with unmethylated, hemi-methylated, or methylated CGAT|GCAA containing DNA probes. Protein dimer concentrations are indicated. Asterisks mark lanes with the same protein concentration. (B,C) EMSA showing CEBPB preferentially binds to ATTGC|GCAAT 10-mer. (B) Purified CEBPB was mixed with four palindromic methylated DNA probes containing the same consensus TTGC|GCAA 8-mer but different nucleotides at the 5′ and 3′ end of the 8-mer. EMSA showing CEBPB preferentially binds to methylated ATTGC|GCAAT. (C) Purified CEBPB B-ZIP domain dimers were mixed with methylated or unmethylated DNA probes with the same sequences as used in B. Both the acrylamide gel and the binding reactions contained 10 mM Mg2+. Protein dimer concentrations are indicated.

This Article

  1. Published in Advance April 16, 2013, doi: 10.1101/gr.146654.112 Genome Res. 23: 988-997

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