CG methylated microarrays identify a novel methylated sequence bound by the CEBPB|ATF4 heterodimer that is active in vivo

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Figure 3.
Figure 3.

The effect of methylation on DNA binding properties of CEBPB|ATF4 heterodimer. Fluorescence intensities at 660 nm from the 180K feature microarray are plotted for all of the 65,536 8-mers in the background TNNNNNNNNA. The 8-mers are color-coded as in Figure 1. Black lines are fitted to the non-CG 8-mers, and colored lines are fitted to the respective 64 8-mers containing the indicated 5-mers with NCGAT|GNN in blue. Effect of methylation on DNA binding of (A) CEBPB-GST and (B) ATF4-GST. (C,D) Comparison of DNA binding of CEBPB-GST|ATF4 heterodimer to the CEBPB-GST homodimer on (C) unmethylated and (D) methylated arrays. (E) Effect of methylation on DNA binding of CEBPB-GST|ATF4.

This Article

  1. Published in Advance April 16, 2013, doi: 10.1101/gr.146654.112 Genome Res. 23: 988-997

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