Figure 7.
Intragenic TL heterogeneity leads to different translation behavior in vivo. (A) Average sedimentation pattern for 204 short/long TL pairs. (B) CRZ1 is an example of a gene with multiple TLs showing distinct sedimentation patterns in a polysome gradient. (C) TATL-seq profile of CRZ1 from fractions 1 and 7. (D) TLs are sufficient to confer the translational behavior predicted from TATL-seq (four of six genes). In vivo translation (ordinate) was determined as luciferase activity per unit RNA. Mean and standard deviation of biological triplicates is shown, (*) P < 0.05, Student's t-test.
