uAUGs are an underrepresented and conserved sequence element associated with decreased translation. (A) TATL-seq sedimentation pattern for uAUG-containing and all TLs. Relative abundance (ordinate) is the abundance of a given TL in a fraction divided by its abundance across the entire gradient. (B) Fold change in mRNA steady-state levels for single TL genes, either with uAUG-containing TLs or with all TLs. Numbers in parentheses indicate number of genes. (C) The fraction of uAUG-containing TLs was calculated based on observed TL lengths for single TL genes (red arrow) and 10,000 randomizations of gene-specific TL length (histogram). P-value based on Z-score of the observed value compared to histogram. (D) Conservation of each of 64 possible uNNN trinucleotides in the TL region was calculated using a genome-wide alignment of S. paradoxus, S. mikatae, S. bayanus, and S. cerevisiae and single TL genes. The ordinate is the number of conserved instances over the total occurrences of that trinucleotide. Near-uAUG trinucleotides are highlighted in blue.
