Roles for transcript leaders in translation and mRNA decay revealed by transcript leader sequencing

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Figure 4.
Figure 4.

Translation-associated TL-seq (TATL-seq) quantifies translation activity of TLs in vivo. (A) TATL-seq was performed on each of seven fractions across a polysome gradient. (B) Peaks were called on the computationally pooled TATL-seq fractions, and then RPKMs were computed for each fraction individually. The Spearman correlation between two gradient fractions is shown. (C) Heatmap of Spearman's ρ for peak abundance in different TATL-seq fractions.

This Article

  1. Genome Res. 23: 977-987

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