Examples of highly occupied and low-complexity regions of transcription factor binding. Binding of 57 transcription factors in one or more developmental stages (98 ChIP-seq data sets in total) were obtained from the C. elegans modENCODE Consortium and are shown for two examples. Data sets are shown in identical order to Supplemental Table 1. (A) Binding proximal to translation elongation factor 1-alpha homolog eef-1A.1. An ∼450-nt HOT region indicated by the red dashed box is significantly enriched for binding in ChIP-seq analyses of 44 different transcription factors. Transcription factor complexity (the number of factors with ChIP-seq binding sites overlapping that position) is shown at the top. (Below) Regions of significant enrichment (Q-value ≤ 10−5) observed in each of the 98 ChIP-seq data sets are shown as black boxes. (B) Low-complexity HLH-1 binding proximal to HLH-1–activated target pat-3 is shown.
