Dynamic, EP300-associated H3K27ac regions grouped into three temporal clusters. (A) Hierarchical clustering heat map of dynamic EP300-associated H3K27ac variants. Cluster names and number of regions per cluster are indicated in the adjacent colored bar. (B,C) Tag density map of dynamic H3K27ac clusters during VEGFA stimulation time course, without (B) or with (C) the EP300 inhibitor C646. Each row represents a 4-kb region centered on a dynamic H3K27ac site. The same color scale was applied within each cluster. Note the loss of VEGFA-induced changes of H3K27 acetylation. (D) H3K27ac aggregation plots, centered on nearby EP300 peaks. Maximal H3K27ac density occurred adjacent to EP300 peak centers. (E) EP300 aggregation plots, centered on EP300 peaks within 2 kb of dynamic H3K27ac regions.
