VEGFA-stimulated dynamic changes in H3K27ac occupancy. (A,B) Browser views of H3K27ac and EP300 ChIP-seq enrichment at HLX and NR4A1 at four time points following VEGFA stimulation. Note areas with varying H3K27ac occupancy in response to VEGFA (boxed regions). Bracketed regions are enlarged in portions of panel C. (C) H3K27ac variance score histogram. VEGFA-stimulated significant changes in H3K27ac regions during the 12-h time course. The plot only includes regions with a log2 variance score greater than or equal to 2. (D) Chip-qPCR validation of VEGFA-induced changes in H3K27ac occupancy. Browser views show ChIP-seq enrichment, and lower plots indicate H3K27ac ChIP-qPCR using numbered amplicons indicated in the browser views. Underlined numbers indicate regions where ChIP-seq predicted VEGFA-stimulated H3K27ac occupancy change, while regions without underline are adjacent controls. Horizontal scale bars: (blue) 1 kb, (green) 0.5 kb.
