HCFC1 is a common component of active human CpG-island promoters and coincides with ZNF143, THAP11, YY1, and GABP transcription factor occupancy

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Figure 6.
Figure 6.

HCFC1 binds ZNF143. (A) Schematic representation of the HCFC1 and ZNF143 proteins and fragments used in the analysis. Features are indicated above the proteins, and HCFC1 fragment names are indicated below. (B) Immunoprecipitation (IP) of extracts from control (lane 3) or Flag-HA–HCFC1N1011 positive cells (lanes 1,2) using Flag (lanes 2,3) or a naive (TBP) antibody (lane 1). The proteins were immunoblotted (WB) using HA or ZNF143 antibodies. (C) GST-pull-down assay using either GFP as negative control (lanes 1,3) or ZNF143 recombinant protein (lanes 2,4) and different in vitro–translated full-length or fragments of HCFC1 as indicated to the left. (D) GST-pull-down assay using different fragments of ZNF143 (full-length [lanes 1,4]; DNA-binding domain [lanes 2,5]) or GFP (lanes 3,6), and the Kelch domain (N380) of HCFC1. (Supernatant) Unbound material after pull-down; (pull-down) material recovered on the glutathione-agarose beads.

This Article

  1. Published in Advance March 28, 2013, doi: 10.1101/gr.150078.112 Genome Res. 23: 907-916

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