Figure 1.
Recovery of C. trachomatis DNA following IMS and IMS-MDA on a serial dilution of C. trachomatis EBs. Post-IMS values are indicated by the gray diamonds and represent total genome copies recovered by IMS. The DNA recovered from the −5 dilution sample was below the detection limit of 1000 genome copies/microliter. Post-IMS-MDA values are indicated by black squares and represent genome copies/microliter. Error bars indicate standard deviation from duplicate experiments. The dotted gray line indicates the cut-off load, below which IMS-MDA produces insufficient DNA for sequencing.
