Figure 2.
Comprehensive mutational scanning of a 100-bp fragment of ARS1 using mutARS-seq. (A) A library of randomly mutagenized ARS1 fragments was cloned into a URA3 vector. Yeast were transformed with these libraries and competed in liquid batch growth. (B) The log2 of the enrichment ratio is shown for all substitution mutations within the ARS1 fragment (top: average; bottom: individual nucleotide substitutions). (Blue) Previously described ACS, B1, and B2 elements. (Red box) A region of nucleotides that repress wild-type ARS1 function. The data shown are the composite of multiple samples as described in the Supplemental Analysis.
